FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094914
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38230.3491598426908415No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30990.28303592793589266No Hit
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA29130.2660482923773009No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG27500.25116127842004027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25650.23426497423541942No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC23420.2138980778399034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22290.203577632581189No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21820.19928505800455562No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA21340.19490115205395125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20790.18987792648555046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20510.1873206480143646No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT19560.17864416748712683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19140.17480824978034804No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA18780.17152032031739478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18370.16777573398458692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17700.16165653192853502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17420.15909925345734915No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17170.15681596910807608No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17080.15599398674233775No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG16570.15133608666982065No Hit
GTATATTACTGTGCGAGACTCGATAGTGGAAACGATCGACACGGCTATTATTACTACTACCACGGTTTGGACGT16460.15033144155614048No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16100.14704351209318722No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15750.14384691400420488No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC15650.14293360026449564No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15540.1419289551508155No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15220.13900635118374594No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG15110.13800170607006576No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14940.13644907271256007No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG14720.13443978248519975No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT14480.13224782950989758No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC14470.13215649813592664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.12987321378665356No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14210.12978188241268263No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14200.12969055103871172No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14170.12941655691679896No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13840.12640262157575846No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13690.12503265096619462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13310.12156205875529952No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA13300.12147072738132858No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCGGTATATTACTGTCAGCATTATGGTGGTTCA13130.11991809402382289No Hit
CTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCA12990.11863945478822993No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12780.11672149593484053No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12560.11471220570748021No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12560.11471220570748021No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12380.11306824097600361No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12090.11041963113084681No Hit
CATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTTCA11950.10914099189525389No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11910.10877566639937018No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11820.10795368403363187No Hit
CTATTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTTTGTCTATTACAATGGGAGAAC11700.10685770754598077No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGAC11680.10667504479803894No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11670.106583713424068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC11540.105396405562446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11410.104209097700824No Hit
GTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGA11380.10393510357891121No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11150.10183448197757998No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACT11010.10055584274198705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)