FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672977
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38370.5701532147458235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30180.44845514779851314No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25670.38143948455890764No Hit
GTCTACAAATTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25460.3783190213038484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25140.37356402967709146No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22910.34042768177812915No Hit
TTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCGTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAAT22510.334483942244683No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22410.3329980073613214No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22130.3288373896879091No Hit
GGTCTAGTCAGAGCCTCCTGCATAGAAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC22060.327797235269556No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21100.3135322603892852No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20840.3096688296925452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19350.28752839993045826No Hit
GTAATAAACTCCAACATCGTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA19210.28544809109375213No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.2851509041170798No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18560.2757895143519021No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18540.2754923273752298No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAA18510.2750465469102213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17870.26553656365670747No Hit
GGCCAAAAGTGTACATGGGAATTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCGTCAGCCTCCACTCTG17830.2649421897033628No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17460.2594442306349251No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17290.2569181413332105No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17050.2533518976131428No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17040.2532033041248066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16620.24696237761468817No Hit
GTTTATTACTGCATGCAAGGTCTACAAATTCCCATGTACACTTTTGGCCA16270.24176160552292278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16130.23968129668621663No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAAGCCAAATAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15810.2349263050594597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15580.23150865482772812No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAAAATCAGCAGAG15180.22556491529428196No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14870.2209585171558612No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCT14690.2182838343658104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14180.21070556646066657No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13890.2063963552989181No Hit
ATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAATTCCCATGTACACT13810.20520760739222887No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13590.20193855064883345No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGCC13460.20000683530046345No Hit
GAAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTT13270.19718355902207654No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13260.1970349655337404No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC12970.1927257543719919No Hit
GAGTGGAGGCTGACGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAA12760.18960529111693267No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12730.1891595106519242No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA12610.18737638879189036No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12290.18262139716513345No Hit
TTATTACTGCATGCAAGGTCTACAAATTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11950.17756921856170418No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11920.17712343809669573No Hit
CAGTAATAAACTCCAACATCGTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA11800.17534031623666188No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11790.17519172274832573No Hit
GATTTTACACTAAAAATCAGCAGAGTGGAGGCTGACGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAATT11680.173557194376628No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACT11440.16999095065656034No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11350.16865360926153494No Hit
ACTATAAGCCGTCCCTCAAGAGTCGCGTCGCCATTTCCGTCGACACGTCCAAGAACCAGTTCTCCCTGAAACTGA11340.1685050157731988No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11300.16791064181985416No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11300.16791064181985416No Hit
CCCCTGGCGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11280.16761345484318185No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11170.16597892647148416No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC11020.16375002414644185No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10870.16152112182139952No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10690.15884643903134876No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10660.1584006585663403No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGC10390.15438863438126416No Hit
CTCCAACATCGTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCACTGAAC10150.15082239066119646No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT10110.15022801670785182No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10010.1487420818244903No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9970.14814770787114567No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9800.14562161856943107No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9710.14428427717440567No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9690.14398709019773334No Hit
CCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCTGCAG9560.14205537484936337No Hit
AGTCAGAGCCTCCTGCATAGAAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG9520.14146100089601873No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9500.14116381391934643No Hit
GTCTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGCCTCCGGGGTCC9500.14116381391934643No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9470.14071803345433795No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9470.14071803345433795No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9430.14012365950099334No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9340.13878631810596798No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGCCTCCGGGGTCCCTGA9340.13878631810596798No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9300.13819194415262334No Hit
GCATAGAAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC9170.13626022880425334No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTATTTGGCTTCTAGT9150.13596304182758104No Hit
GTCTCTGGTGCCTCCGTCAGCAGTCCTAATTATTTTTTGGGCTGGATCCG9140.1358144483392449No Hit
CTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCTGCAGGTCTA9070.1347742939208918No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9050.1344771069442195No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8990.13358554601420256No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8910.13239679810751334No Hit
CTCCACAGCTCCTGATTTATTTGGCTTCTAGTCGGGCCTCCGGGGTCCCT8890.13209961113084104No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8870.1318024241541687No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTTCTATGC8850.1315052371774964No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8810.1309108632241518No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8750.1300193022941349No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8710.12942492834079025No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8580.12749321299242022No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8560.12719602601574795No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8410.12496712369070562No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8370.124372749737361No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8330.1237783757840164No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8320.12362978229568025No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8260.12273822136566331No Hit
GGCACAGATTTTACACTAAAAATCAGCAGAGTGGAGGCTGACGATGTTGGAGTTTATTACTGCATGCAAGGTCTA8250.12258962787732716No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8230.12229244090065484No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8230.12229244090065484No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8150.12110369299396563No Hit
GTGGAGGCTGACGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAT8080.12006353857561254No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGGC8070.11991494508727639No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG8030.11932057113393177No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7990.11872619718058715No Hit
GCGTATTACTGTGCGAGACAGTCCTCTGGAGAAGCGGATATCTGGGGCCG7980.118577603692251No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7960.11828041671557868No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7930.11783463625057024No Hit
GTAATACGCAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGA7730.11486276648384715No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTC7710.11456557950717484No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCGCCAGGGGTGACGGGCAGG7710.11456557950717484No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7520.11174230322878792No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCA7510.11159370974045177No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7490.11129652276377945No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7370.10951340090374559No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7300.10847324648539253No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGG7290.10832465299705638No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7290.10832465299705638No Hit
AAAGTGTACATGGGAATTTGTAGACCTTGCATGCAGTAATAAACTCCAAC7140.10609575067201404No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7090.10535278323033329No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7030.10446122230031636No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7020.1043126288119802No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6890.10238091346361021No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAT6850.10178653951026559No Hit
ATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGACTAGAAGCCAAATAAATCAGGAG6790.10089497858024865No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTG6770.10059779160357635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1250.057.9150541
TGGTATC1350.053.617082
GGTATCA4600.047.9627761
CTTTGCG306.3383864E-445.957514
GTATCAA12750.043.5309221
GAATCTA405.020541E-543.0947621
CGAACCT602.700108E-740.6324853
TATCAAC14000.039.3921432
ATACTAT350.001353556939.392146
CGGGGAC952.2191671E-1036.28223417
ATCAACG15200.035.828713
TCAACGC15500.035.1352464
ATAGCGG601.1905489E-535.10724363
CAACGCA15700.034.9072075
ACGAACC709.0769936E-734.8069352
AACGCAG15850.034.576856
TACTCCG601.3502437E-534.4681245
TCCGGTC601.3509309E-534.465568
AGAGTAC16900.033.03799411
GACGGAA3750.033.03049570
Produced by FastQC (version 0.11.5)