FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368834
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGT200.007400232451.669984
AATCCGA558.748766E-443.03315770
GTATCAA12650.041.3960571
GGTATCA5000.039.2745061
CAACGCA14500.037.0598455
ATCAACG14700.036.555633
TCAACGC14700.036.555634
TATCAAC15100.035.815392
AACGCAG15050.035.7054986
ATGATTA400.002446173734.9152852
TCTCGTA501.8374187E-434.56850438
CGCAGAG15550.034.557418
GTCTTAA501.8744617E-434.4513241
GTAACTC400.002613818734.446653
ACGCAGA15900.033.7967157
AGAGTAC16150.033.06025311
CAGAGTA16250.032.85680810
GAGTACG13850.031.83517312
GCAGAGT16900.031.7969079
AGTACGG13800.031.70090313
Produced by FastQC (version 0.11.5)