FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368834
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8010.21717086819544834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT200.00747808151.5333920
GTATCAA13850.043.5394741
GGTATCA5200.041.0849951
GTATAGA350.001354637139.381011
TCAACGC15800.037.829514
TATCAAC16300.037.166162
CAACGCA16100.037.1246155
ATCAACG16350.036.5817573
AACGCAG16600.035.799476
AGAGTAC17350.034.44993211
CATATAC501.8992946E-434.3742373
ACGCAGA17300.034.3509377
CGCAGAG17550.033.8616078
CAGAGTA17900.033.39141510
GCAGAGT18450.032.209829
AATAGCG901.6331614E-731.3633262
TGGTATC2000.030.9788422
CGGCTTT450.004708152730.54658926
GGAGCGT450.00471753330.5341647
AGTACGG14800.030.17311913
Produced by FastQC (version 0.11.5)