FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909369
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAG21430.23565791224464439No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG20970.23059945962530062No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAAT20340.2236715788640255No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18690.2055271292511621No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGG18210.20024874390923816No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATT15900.17484651445122937No Hit
GATTACTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT15860.17440664900606903No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14940.16428974376738156No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14340.15769176208997668No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13820.15197351130289244No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC13810.15186354494160237No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13170.14482569781903715No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGT12060.13261943171583812No Hit
GAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAG11700.1286606427093952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11460.12602145003843324No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAG11030.12129289650295975No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10760.11832380474812755No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9960.10952649584492102No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9950.10941652948363095No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9830.10809693314814998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9610.10567767319976817No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9580.10534777411589794No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9530.10479794230944753No Hit
CTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC9400.10336837961267649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)