FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852925
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACG350.007688607651.17531670
GTATCAA21900.042.320141
TCGTATG602.8230897E-740.40474340
GGTATCA8950.037.3411451
TATCAAC25800.035.6516462
TCAACGC26200.034.9758574
ATCAACG26400.034.7108843
CAACGCA26850.034.1291435
CTCGTAT751.6222875E-632.3161839
AACGCAG28500.032.1532446
TGGTATC4000.031.8661632
GTGGTAT4250.031.616561
ACGCAGA30100.030.327877
CGTATGC802.6644375E-630.32500641
CAGAGTA30750.029.91084510
AGAGTAC30550.029.76838511
CGCAGAG30700.029.7351468
GCAGAGT33250.027.6619079
TAAGCGC500.007782167327.56477223
CTTATAC1208.623647E-825.8404561
Produced by FastQC (version 0.11.5)