FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852925
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.040.4672661
GTGGTAT4800.036.6266251
GGTATCA11250.035.8509981
TGGTATC5000.035.128532
ATCAACG29050.034.7116663
TCAACGC29150.034.4522864
CAACGCA29150.034.448255
TATCAAC30100.033.638812
AACGCAG30250.033.0818986
ACGCAGA30650.032.5379647
CGCAGAG31500.031.6599528
AGAGTAC32100.031.49670611
CAGAGTA32450.031.0510110
CGAGTTA450.00467110430.60052126
TAACGGC1151.6825652E-930.03399536
GCAGAGT35000.028.6904689
CACGATT500.006945753528.21436963
TTAACGG1306.2627805E-926.55446435
ACGGGTA2350.026.34225716
AGTACGG31100.025.9854513
Produced by FastQC (version 0.11.5)