FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464093
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40750.040.3013731
GGTATCA17150.038.3440781
ATCAACG47500.034.3557133
TCAACGC47400.034.355564
TATCAAC48050.034.1774182
CAACGCA47650.034.1753125
AACGCAG49000.033.3040056
ACGCAGA51600.031.6248197
AGAGTAC52350.031.23750711
CGCAGAG52950.030.7535028
CAGAGTA54900.029.91210
AGTACGG42850.028.92549113
GCAGAGT57100.028.6389399
GAGTACG43800.028.29714412
TGGTATC6850.028.1456762
GTGGTAT7100.027.6404781
GTACGGG46300.026.54705214
TACGGGG18450.026.12526715
TACGGGT4950.024.34399815
ACGGGTA2001.8189894E-1224.10055716
Produced by FastQC (version 0.11.5)