FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464093
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41750.040.863531
GGTATCA17550.037.706071
TATCAAC47600.036.21882
TATCGGC400.00229569935.3771364
CAACGCA48300.035.1398855
TCAACGC48450.034.966154
ATCAACG48950.034.76723
AACGCAG49600.034.3574146
GTGGTAT6850.033.2078361
TGGTATC6900.031.917892
ACGCAGA53850.031.8372347
AGAGTAC54800.031.41070611
CGCAGAG55800.030.7246428
CAGAGTA57650.029.9770710
GCAGAGT59400.029.0939049
AGTACGG47000.027.85143913
GAGTACG47650.027.47151212
GTACGGG51550.024.79332414
TACGGGG19650.024.4819115
AATAGCG3200.023.14299862
Produced by FastQC (version 0.11.5)