FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18745
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT690.36809815950920244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.22939450520138704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.20805548146172315No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.19738596959189117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.19738596959189117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1920512136569752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1920512136569752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.18138170178714325No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.18138170178714325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.18138170178714325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.13336889837289945No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG250.13336889837289945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250.13336889837289945No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.12803414243798347No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT230.1226993865030675TruSeq Adapter, Index 11 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1173646305681515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1173646305681515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10669511869831955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10136036276340357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10136036276340357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTT150.001626216875.4093855
ATATCAA254.4341796E-664.521391
TGGTCTT200.00431264658.95195466
GGGGGGG951.8329549E-434.141270
TACGGGA554.6489327E-429.32790615
TACGGGG607.7343406E-426.88391515
GAGTACG2600.026.05671712
TATCAAC2750.025.8085562
AACGCAG2900.025.586076
AGTACGG2650.025.56507913
GTACGGG2650.025.56507914
GTATCAA2350.023.3375241
ATCAACG3250.022.8306473
CAACGCA3250.022.8306475
TCAACGC3400.021.8234124
ACGCAGA3550.020.9012977
TGGTATC800.004125009820.1629352
GTGGTAT800.004125009820.1629351
CAGAGTA3700.020.05394610
AGAGTAC3750.019.78655811
Produced by FastQC (version 0.11.5)