FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18745
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1490.7948786343024806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT320.17071218991731127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1653774339823953No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.15470792211256335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.14937316617764737No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250.13336889837289945No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC240.12803414243798347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10669511869831955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10669511869831955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTG150.002512792867.6136930
GTATGCG200.00864608549.489975
TATGCGT200.00864608549.489976
ATGCGTG200.00864608549.489977
CGTATGC200.00864608549.489974
CGGGTGC601.729003E-532.99331317
GGGTGCC601.729003E-532.99331318
GGTGCCT601.729003E-532.99331319
ACGGGTG851.6340891E-731.05253416
TATCAAC3500.030.1653182
GTATCAA3450.029.7256491
GTGCCTG704.9561764E-528.27998420
AACGCAG3650.028.0217236
CAGAGTA3650.028.02172310
AGAGTAC3650.028.02172311
GCAGAGT3650.028.0217239
AGTACGG3200.027.8381113
GAGTACG3200.027.8381112
TACGGGT1203.772584E-927.49442915
GTACGGG3300.026.99453414
Produced by FastQC (version 0.11.5)