FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014172
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60430.5958555353529775No Hit
GAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAACCTGGGAAAG44980.4435145123312416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44860.44233128108447084No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT42380.41787783531787503No Hit
GTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA41480.40900360096709437No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37740.3721262271094055No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36280.3577302469403612No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36110.3560540026741026No Hit
ACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA34310.33830553397254115No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34020.3354460584595118No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33930.3345586350244337No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31190.3075415215565013No Hit
GAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT30890.3045834434395744No Hit
GAGTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30020.2960050169004863No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30010.29590641429658876No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28880.28476432005616403No Hit
GTCTCCATCCTCCCTGTCTGCATGTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGG27670.2728334049845588No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27380.2699739294715295No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26890.2651424018805489No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26570.2619871185558268No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26170.2580430143999243No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25600.25242266597776314No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25490.25133803733488996No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25460.25104222952319727No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25450.25094362691929967No Hit
GGCCAAAAGTGTACGGAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCA25330.2497603956725289No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24480.241379174341236No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23790.23457559467230413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22870.22550415511372823No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22440.22126424314613302No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22280.219686601483772No Hit
GTCTGCATGTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGA21750.21446066347720108No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21500.211995598379762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.20785428901606431No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACT20790.204994813503035No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20640.20351577444457153No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20320.20036049111984952No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20260.19976887549646413No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20010.19730381039902503No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA19440.1916834619768639No Hit
AGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAACCTGGGAA18210.17955534169746354No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18180.17925953388577084No Hit
GTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGC18040.17787909743120495No Hit
ACTTACTACTGTCAACAGAGTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18010.17758328961951225No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17730.17482241671038048No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17590.1734419802558146No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17280.17038529953499013No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC17160.16920206828821935No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17070.1683146448531413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17010.1677230292297559No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC16950.16713141360637052No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT16440.16210268080759477No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16330.16101805216472156No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16010.15786276883999953No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT15920.15697534540492145No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG15850.1562851271776385No Hit
GTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAA15480.15263683083342863No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15310.15096058656717007No Hit
ACCTTGATGGGACCCCACTTTGCAAACTAGATGCACCAAAGATCAAGAACTTAGGGGCTTTCCCAGGTTTTTG14800.14593185376839432No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14690.14484722512552112No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14310.1411003261774137No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14280.140804518365721No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATGTGTAGGGGACAGAGTTACCATCA14220.1402129027423356No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13970.1377478376448965No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13820.13626879858643307No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG13780.13587438817084282No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACTCCGTACACTTTTGGCCAGGGGA13720.13528277254745744No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13690.13498696473576474No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13680.13488836213186717No Hit
CCATCCTCCCTGTCTGCATGTGTAGGGGACAGAGTTACCATCACTTGCCG13650.13459255432017447No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13640.13449395171627693No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13570.133803733488994No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13540.1335079256773013No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13490.13301491265781348No Hit
GTAATAGACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA13280.13094425797596465No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13120.1293666163136036No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13020.12838059027462798No Hit
GCCCCTAAGTTCTTGATCTTTGGTGCATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT12990.12808478246293528No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12890.12709875642395962No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12810.12630993559277914No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12790.12611273038498402No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12780.12601412778108645No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12730.12552111476159863No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12710.1253239095538035No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12600.1242392809109303No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG12450.12276024185246684No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12410.12236583143687658No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12220.12049238196282287No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12170.11999936894333506No Hit
ATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12090.11921054811215455No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC12040.11871753509266672No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11860.11694268822251058No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11860.11694268822251058No Hit
GTCTATTACTGTGCGAGACAAGTTGAATATGGTGACAACGAGCGTTTCGT11760.11595666218353494No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11670.11506923874845687No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11670.11506923874845687No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11520.1135901996899934No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11250.1109279293847592No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11240.11082932678086162No Hit
GTTCTTGATCTTTGGTGCATCTAGTTTGCAAAGTGGGGTCCCATCAAGGT11100.10944889032629573No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11070.10915308251460305No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT10780.1062936070015737No Hit
GGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATT10680.10530758096259805No Hit
ATTTAAATTGGTATCAGCAAAAACCTGGGAAAGCCCCTAAGTTCTTGATC10500.1035327340924419No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10440.10294111846905654No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10430.10284251586515897No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10360.10215229763787602No Hit
CTAGATGCACCAAAGATCAAGAACTTAGGGGCTTTCCCAGGTTTTTGCTG10240.10096906639110526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT350.00730828351.83737670
TACGGAT4000.048.3815570
GTGGTAT2250.044.4143681
GTATCAA17150.043.6017151
TTAACGG350.001339212539.47952335
TATCAAC19200.038.9444052
ATCAACG19300.038.742623
TCAACGC19400.038.542914
CAACGCA19600.038.3254245
ATCTCCG655.505117E-737.10648310
AACGCAG20350.036.912946
GACGGTA450.001984440736.4415569
CGCAGAG21550.035.175528
CTACGGA4000.034.84723369
AGAGTAC21700.034.45602411
ACGCAGA22900.033.1018527
GCAGAGT22900.032.951399
CCTATAC553.2802613E-431.32523
TATACAC1101.0732037E-931.32523
AGTACGG19100.031.02845813
Produced by FastQC (version 0.11.5)