FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263160
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTG150.002137595370.6942363
GTGGTAT1750.047.1641271
GTATCAA11600.043.5810781
TGGTATC1950.040.501412
GGTATCA4800.038.6893231
TATCAAC13250.038.0959172
AACGCAG14850.034.4079366
ATCAACG14700.034.312043
TCAACGC14800.034.0607724
CAACGCA14900.033.8321725
ACGCAGA15600.032.7537087
CGCAGAG15700.032.545098
AGAGTAC16100.031.73651311
CAGAGTA16500.031.17497810
GCAGAGT16900.030.2341949
ACGGGAC802.8877203E-630.00021216
TACGGGG3900.029.01119415
TAATACT953.3608376E-728.8778724
TATAAGC500.0079035227.4705222
AGTACGG12500.026.60590413
Produced by FastQC (version 0.11.5)