FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745032
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41540.5575599437339605No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC32380.4346122045764477No Hit
GTATAATGGTTATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT31600.4241428556088866No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGAGGCGT29620.3975668159220007No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT29130.3909899172116097No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26160.3511258576812808No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25720.34522007108419506No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24980.33528761180727806No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24830.3332742754673625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24660.3309924942821248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24440.3280396009835819No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATAA22760.3054902339765272No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22290.2991817801114583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19430.26079416723040083No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18940.25421726852000986No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAA18560.24911681645889033No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18410.24710348011897473No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA17850.23958702444995653No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17700.2375736881100409No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC17350.2328759033169045No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17160.23032567728634473No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17060.22898345305973436No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA16950.2275070064104629No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16890.22670167187449664No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16760.22495678037990313No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16560.22227233192668233No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16140.21663499017491866No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTG15920.2136820968763758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15700.2107292035778329No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15620.2096554241965446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15580.2091185345059004No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15560.20885008966057833No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATAACCATTATA15500.20804475512461207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15230.204420749712764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15100.20267585821817047No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA14080.1889851711067444No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13840.18576383296287946No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATCTGCAGATAGA13700.1838847190456249No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13260.17797893244853913No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13040.17502603914999623No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAACCACATGACCCAGTCTCCTTCCACC12940.17368381492338583No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCA12710.17059669920218193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12680.1701940319341988No Hit
AGTTTAGGAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC11830.1587851260080104No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11770.15797979147204416No Hit
ACCTTGAAGGGACCCCACTTCCTAAACTAGACGCCTCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTG11510.1544900084828571No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC11490.15422156363753503No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11450.15368467394689087No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11150.14965800126705966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11130.14938955642173757No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10900.14630244070053366No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC10750.14428910436061806No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10720.14388643709263496No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA10550.14160465590739726No Hit
CAGTATAATGGTTATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT10200.13690687111426086No Hit
CCACATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAG10200.13690687111426086No Hit
GATCTATGAGGCGTCTAGTTTAGGAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10190.13677264869159983No Hit
GGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGAGGCGTCTAGTTTAGGAAGTGGGGTCCCTT10130.1359673141556336No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9890.13274597601176863No Hit
GTTTAGGAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC9750.13086686209451406No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9640.1293904154452426No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9630.1292561930225816No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCC9630.1292561930225816No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC9540.1280481912186322No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9540.1280481912186322No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9530.1279139687959712No Hit
GTGTATTACTGTGCGAGAGATGCTCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGC9390.12603485487871663No Hit
TCCTAAACTAGACGCCTCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTT9340.1253637427654114No Hit
GATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACC9280.12455840822944517No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8910.11959217859098668No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8890.11932373374566461No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8860.1189210664776815No Hit
TCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTAT8830.11851839920969838No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.11851839920969838No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8750.11744461982841005No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8710.11690773013776588No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8650.11610239560179965No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG8630.11583395075647758No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8610.11556550591115548No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC8570.11502861622051135No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8560.1148943937978503No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8510.11422328168454508No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8330.11180727807664638No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8310.11153883323132428No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8280.11113616596334117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT8280.11113616596334117No Hit
TCTATGAGGCGTCTAGTTTAGGAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8190.10992816415939181No Hit
GGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCACTTCCTAAA8150.10939127446874765No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA8110.10885438477810348No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8050.10804905024213725No Hit
ACTTATTACTGCCAACAGTATAATGGTTATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA8030.10778060539681517No Hit
GGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCACTTCCTAAACTAGA8010.1075121605514931No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT7990.10724371570617101No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACAACCACATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA7980.10710949328350997No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCACTTCCTAAACT7970.10697527086084893No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7870.10563304663423853No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7870.10563304663423853No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7830.10509615694359437No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAACCACATGACCCAGTC7800.10469348967561125No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7700.10335126544900085No Hit
GTGCCAAATGTGACAACCACATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA7690.1032170430263398No Hit
CCTCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA7680.10308282060367877No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7640.10254593091303461No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7580.10174059637706835No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7560.10147215153174628No Hit
CTCCTGATCTATGAGGCGTCTAGTTTAGGAAGTGGGGTCCCTTCAAGGTT7530.10106948426376317No Hit
GTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGAGG7530.10106948426376317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2850.049.590741
GTATCAA17500.042.5478171
TATCAAC20350.036.557062
ATCAACG20900.035.561573
TCAACGC20950.035.2982334
CAACGCA21200.034.87735
AACGCAG21500.034.3906366
ACGCAGA22200.033.4611637
CGCAGAG23000.032.2972958
AGAGTAC22750.032.19870811
GCAGAGT23300.031.586259
GTTCGGC553.1911954E-431.47120940
GAGTACT5550.030.98255712
AGTACTT5750.029.90490213
AAGTTCG703.8073584E-529.61882638
AGTTCGG605.338094E-428.8252439
TGTTAGA7600.028.04365770
ATGTCTA500.00785393727.5125111
ACTTTTT6500.026.98523516
ACTAAGT658.637986E-426.54698435
Produced by FastQC (version 0.11.5)