FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408438
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.042.5586051
GGTATCA5400.040.2351
TATCAAC16700.035.9327162
CAACGCA16700.035.9327165
TCAACGC16750.035.825454
ATCAACG17050.035.195093
AACGCAG17400.034.685356
ACGCAGA18450.032.5244647
CAGAGTA18650.032.17567410
AGAGTAC18700.032.08964511
ACGGGTA1305.456968E-1231.83428816
CGCAGAG19150.031.3355798
GCAGAGT19300.031.270739
TATACTG854.431804E-628.4011785
AGTACGG16300.027.29350713
GAGTACG16450.027.04462812
TACGGGT2600.025.20214515
GAGTACT6250.024.83074412
TACGGGC4050.024.69449815
AGTACTT6350.024.43970913
Produced by FastQC (version 0.11.5)