FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408438
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.042.4912451
GTATCAA13750.039.9224551
TGGTATC2400.038.7824862
GTGGTAT2450.035.228711
ACGTTAC400.002627822334.41001516
CAACGCA15950.034.086415
ATCAACG16200.033.5891723
TCAACGC16300.033.3585784
AACGCAG16550.032.8506476
TATCAAC16650.032.7134252
ACGCAGA17250.031.517587
AGAGTAC18100.030.22758311
CAGAGTA18300.029.70919210
CGCAGAG18500.029.3880128
GCAGAGT18750.029.1796919
TACGGGT2550.026.98824915
GAGTACG16200.025.48890112
AGTACGG16150.025.3547513
GTATTAA700.001326949224.6600991
TGTTCGT750.001901971223.18124444
Produced by FastQC (version 0.11.5)