FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195904
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAA200.007335724351.77534533
CTCTTAG306.3071784E-445.987211
GGTATCA2600.042.4497341
CTATACT651.1561497E-842.3522224
ACTATAC503.874389E-641.3250583
GTATCAA5650.039.6792261
TACCGCG451.01380094E-438.2346428
CGGTACC400.002492060734.7750845
TATCAAC6450.034.7222442
ATCAACG6450.034.7045063
TCAACGC6450.034.6779334
GTATTAG807.0602255E-834.4904061
TACTATA501.8700342E-434.4551472
GGTACCG501.8841276E-434.411186
AGGGTAC400.002624064234.411184
CAACGCA6600.033.8897975
AACGCAG6750.033.6464846
TACGGGG2600.033.0961215
ACGCAGA6900.032.915047
CGCAATC552.894089E-431.98495962
Produced by FastQC (version 0.11.5)