FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025124
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53120.5181812151505574No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48120.46940662788111487No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG43440.42375361419691665No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42450.41409624591756705No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT42140.4110722215068616No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36530.35634713459054707No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35930.350494184118214No Hit
GATTATTACTGCTGCTCATGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGT34580.3373250455554645No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34350.33508141454107015No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG31740.3096210799864212No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGC30780.30025635923068816No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCA29970.2923548760930385No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCA29910.2917695810458052No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28670.2796734834029834No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28160.27469847550150034No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27430.2675773857601617No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25090.2447508789180626No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24910.24299499377636266No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24800.24192195285643492No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC24500.23899547762026838No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24130.23538615816232963No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23750.231679289529852No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23490.229143010991841No Hit
GCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAG22940.22377780639220232No Hit
CGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGT22620.22065623280695798No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC22250.21704691334901924No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC22080.2153885773818582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22050.21509592985824152No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21780.2124621021456916No Hit
GGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG21590.2106086678294528No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20850.20339002891357535No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC20000.1950983490777701No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19440.18963559530359253No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTCCTCAACTCCATGTAGG19290.18817235768550927No Hit
GGTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTC19190.18719686594012042No Hit
GTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCA18290.17841744023162076No Hit
ACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC18220.17773459600984856No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18190.1774419484862319No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17950.17510076829729868No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAA17370.16944291617404333No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG17340.16915026865042668No Hit
GACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAAT17330.1690527194758878No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17180.16758948185780453No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG17010.16593114589064348No Hit
GTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT16820.16407771157440468No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC16640.16232182643270474No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16600.1619316297345492No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16510.16105368716369922No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16310.1591027036729215No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16260.15861495780022708No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15990.1559811300876772No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15930.15539583504044388No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT15890.15500563834228834No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAAGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAA15880.15490808916774945No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15800.15412769577143837No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA15410.15032327796442188No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT15300.14925023704449414No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15060.14690905685556088No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14680.14320218822308325No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14340.13988551628876117No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC14230.13881247536883343No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14230.13881247536883343No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG14120.13773943444890568No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13750.13413011499096697No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13700.1336423691182725No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13280.12954530378763934No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC13200.12876491039132826No Hit
GTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTT13120.1279845169950172No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13040.12720412359870611No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGG12960.12642373020239503No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12920.1260335335042395No Hit
ATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAA12920.1260335335042395No Hit
TGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGT12910.1259359843297006No Hit
CAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCG12830.12515559093338952No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12810.12496049258431176No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12720.12408255001346179No Hit
CTTATTGACCTCATAAATCATGACTTTGGGGGCTTTGGCTGGGTGCTGTTGGTACCAGGAGACAAGGTTATAACC12680.12369235331530626No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12560.12252176322083963No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG12550.12242421404630074No Hit
GTCTATTACTGTGCGAGAACCTATTATTATGGTTCGGGGAGTCCACCAGT12550.12242421404630074No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12540.12232666487176184No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC12480.12174136982452854No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC12380.12076587807913969No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12370.12066832890460082No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG12240.11940018963559532No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12220.11920509128651754No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12180.11881489458836199No Hit
GTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCC12100.1180345011920509No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12000.11705900944666207No Hit
GCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGG11920.11627861605035099No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11850.11559577182857879No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG11800.11510802595588437No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11420.11140115732340672No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11380.11101096062525119No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAG11360.11081586227617343No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTGCTCATGT11280.11003546887986233No Hit
CTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAG11250.10974282135624569No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC11180.10905997713447349No Hit
GGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAA11160.10886487878539572No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT11120.10847468208724019No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11110.10837713291270129No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11070.10798693621454575No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11050.10779183786546799No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11020.10749919034185133No Hit
GTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA10950.10681634612007913No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAAC10930.10662124777100136No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC10930.10662124777100136No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA10910.1064261494219236No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10820.10554820685107363No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10810.10545065767653475No Hit
GCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTAC10750.10486536262930143No Hit
CTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC10600.10340212501121816No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10500.1024266332658293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2400.051.699372
GTGGTAT2450.049.23751
CGTATGC451.8141891E-646.17119241
TCGTATG451.8172541E-646.15987840
GGTATCA7750.045.362031
CGCGGTA7750.043.62568370
TCTCGTA503.773357E-641.5093238
TGTAATA503.86833E-641.3594974
GTATCAA19900.041.220941
TATCAAC22750.035.7539982
TCAACGC23150.035.4339834
ATCAACG23250.035.2815743
AATGGTA750.002530883334.67682670
CTCGTAT601.3161667E-534.5995739
ATCTCGT601.3205872E-534.58263837
AACGCAG23950.034.394296
AGCGTCA1154.7293724E-1132.9677163
AGAGTAC25150.032.75321211
CGCAGAG25050.032.6087848
CAGAGTA25450.032.36712310
Produced by FastQC (version 0.11.5)