FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025124
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50430.4919404871995973No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45580.44462913754823813No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42080.4104869264596283No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT41660.40638986112899517No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40920.3991712222131176No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35750.3487382989765141No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35640.3476652580565863No Hit
GATTATTACTGCTGCTCATGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGT33680.32854561984696484No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33050.3224000218510151No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31580.30806029319379896No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGC30130.2939156628856607No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG29400.28679457314432205No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCA28680.27977103257752234No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCA28230.2753813197232725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27810.2712842543926393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27480.2680651316328561No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25740.2510915752630901No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24990.24377538717267377No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23490.229143010991841No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23200.22631408493021332No Hit
GCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAG22500.21948564271249138No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22280.2173395608726359No Hit
CGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGT21880.2134375938910805No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC21770.21236455297115275No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21630.21099886452760835No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC21570.21041356948037507No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21400.208755233513214No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20790.202804733866342No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20550.20046355367740876No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20320.19821992266301444No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19910.19422040650692016No Hit
GGTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTC18930.18466058740210942No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18190.1774419484862319No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC18020.17578361251907085No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAA17960.17519831747183756No Hit
GGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG17930.1749056699482209No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17930.1749056699482209No Hit
GTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCA17600.1716865471884377No Hit
ACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC17350.16924781782496556No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTCCTCAACTCCATGTAGG17260.16836987525411562No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17080.16661399011241568No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16990.1657360475415657No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG16840.16427280992348242No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16510.16105368716369922No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16450.1604683921164659No Hit
GACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAAT16370.15968799872015482No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16260.15861495780022708No Hit
GTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT16240.1584198594511493No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16180.15783456440391602No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15840.15451789246959394No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAAGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAA15780.15393259742236062No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15210.14837229447364417No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA15160.14788454860094974No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15050.146811507681022No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14840.1447629750157054No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT14690.14329973739762214No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT14600.14242179482677217No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC14600.14242179482677217No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG14510.1415438522559222No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14250.1390075737179112No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13970.13627619683082243No Hit
CAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCG13830.13491050838727803No Hit
GTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTT13560.13227668067472814No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC13520.13188648397657257No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTGCTCATGT13420.13091099223118374No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13250.1292526562640227No Hit
GTCTATTACTGTGCGAGAACCTATTATTATGGTTCGGGGAGTCCACCAGT13180.1285698120422505No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13140.12817961534409497No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG13080.12759432029686163No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13040.12720412359870611No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12800.12486294340977287No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC12660.12349725496622849No Hit
GTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCC12530.12222911569722296No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12420.12115607477729523No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG12290.11988793550828973No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12280.11979038633375085No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12280.11979038633375085No Hit
ATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAA12250.11949773881013419No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG12180.11881489458836199No Hit
CTTATTGACCTCATAAATCATGACTTTGGGGGCTTTGGCTGGGTGCTGTTGGTACCAGGAGACAAGGTTATAACC12130.11832714871566757No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12020.11725410779573983No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11990.11696146027212317No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11950.11657126357396763No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC11930.11637616522488987No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11870.11579087017765655No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC11830.11540067347950102No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11610.11325459163964555No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11380.11101096062525119No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG11370.1109134114507123No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC11310.11032811640347899No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11170.1089624279599346No Hit
GGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAA11100.1082795837381624No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTC11050.10779183786546799No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11020.10749919034185133No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGG11010.10740164116731245No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10820.10554820685107363No Hit
GCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGG10740.10476781345476255No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10680.10418251840752922No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT10670.10408496923299035No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAAC10630.1036947725348348No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA10590.10330457583667928No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC10580.10320702666214039No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10570.10310947748760149No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10560.1030119283130626No Hit
CTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC10520.10262173161490708No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGT10430.1017437890440571No Hit
GTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGACGACG10280.10028055142597384No Hit
TGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGT10280.10028055142597384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT252.489888E-455.54206545
TCCGTAG252.4983732E-455.50382644
GTGGTAT3300.052.2393721
TTGACGC200.00732763251.80558435
TGGTATC3600.049.7529072
CTCCGTA306.157587E-446.23045743
CGTTTCA306.384554E-445.89215518
GTATCAA22550.041.740531
GGTATCA8800.040.354911
CGGGATT350.001363717639.3342117
TATCAAC25400.037.0208632
ATCAACG27000.034.810043
CGTAGTC400.002515077634.72403746
TCAACGC27300.034.541824
TAATACA400.002627516434.4157564
GACGTTT400.002628144834.41407816
TGAGACG400.002628773734.412413
AACGCAG27700.034.2881666
GCTTGAC1056.475602E-1032.8361781
CGCAGAG29700.032.2109348
Produced by FastQC (version 0.11.5)