FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286713
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46110.3583549711551838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43300.3365163793324541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38050.29571473980600177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34230.2660266897124689No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33830.26291799336759636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31440.2443435327069828No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29650.23043211656367815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28260.219629396765246No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28230.21939624453938059No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28180.2190076574962715No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG28150.21877450527040607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27310.2122462429461737No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26990.20975928587027565No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26700.20750548102024305No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG26540.20626200248229404No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26080.20268700168569062No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25960.20175439278222884No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA25850.20089950128738884No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23000.1787500398301719No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22690.1763408001628957No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22660.17610764793703024No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21190.1646831888696236No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT20860.16211851438510375No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA20670.16064188362128928No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20550.15970927471782753No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG20220.15714460023330767No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20180.15683373059882041No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT19720.15325872980221697No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18730.1455647063486574No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18670.14509840189692652No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG18190.14136796628307946No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA17930.1393473136589123No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT17590.1367049217657706No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17170.13344079060365444No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC16870.13110926834500003No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16860.1310315509363782No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16680.12963263758118557No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC16630.12924405053807647No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT16510.12831144163461472No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT16500.1282337242259929No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG16470.12800057200012746No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16300.1266793760535566No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16130.1253581801069858No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15860.1232598100741968No Hit
ATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGAC15850.12318209266557499No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA15600.12123915745002964No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15340.11921850482586249No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA15300.11890763519137523No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15290.11882991778275342No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15290.11882991778275342No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT15160.11781959147066984No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT14730.11447774289993186No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14730.11447774289993186No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14700.1142445906740664No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14510.11276795991025194No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14480.1125348076843865No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14460.11237937286714286No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT14410.1119907858240338No Hit
CCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGAT14290.11105817692057204No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14250.11074730728608477No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13990.10872665466191761No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13950.10841578502743035No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13900.1080271979843213No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13900.1080271979843213No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13660.10616198017739775No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTCCTCC13560.10538480609117963No Hit
GAAGTGGATCTGGGGCAAATTTTACCCTCACCATCAGCAGCCTGCAACCT13380.10398589273598698No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13350.10375274051012152No Hit
GGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGG13290.10328643605839063No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13210.10266469678941613No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12930.10048860934800535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27600.039.2599141
TATCAAC29550.036.433512
ATCAACG30200.035.649343
TCAACGC30550.035.2409254
CAACGCA31300.034.396495
AACGCAG32250.033.4899186
CGCAGAG33700.032.148528
AGAGTAC34600.031.41169511
CGTATGC1001.4037141E-831.15418841
GTGGTAT3100.031.0702131
CAGAGTA35150.031.01803810
ACGCAGA35900.030.0826157
GCAGAGT36650.029.6546979
GTACTAG703.9290546E-529.4849991
TCGTATG1103.5695848E-828.30317140
GACAGGT3300.028.144771
TGTTAGA13600.027.5669370
ACGGGTA1005.1502684E-727.51826116
AGTACGG31600.026.55725313
GAGTACG31900.026.41531612
Produced by FastQC (version 0.11.5)