FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202559
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC51160.4254261121491752No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50240.41777575985876786No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49360.41045803158098687No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45540.378692438375165No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT43020.3577371255797013No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41340.3437669170493922No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39270.3265536243959756No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35850.29811427131641777No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34980.2908796990417934No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG33960.28239778671982No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC33690.28015257463459176No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33440.27807367455567666No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG31950.265683430085343No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC31900.26526765006956No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT30880.25678573774758656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30680.25512261768445454No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC30670.255039461681298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30390.2527110935929131No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG30230.2513805975424075No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGAC29300.2436470892488435No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC29230.24306499722674732No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTC28740.23899035307207378No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA28160.2341673048889909No Hit
GATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTA27280.2268495766112099No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26780.22269177645337984No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25600.2128793680809008No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25340.21071731199882918No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24760.20589426381574624No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTA24670.20514585978733685No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA24500.20373220773367462No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT22910.1905104032317749No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21980.18277689493821095No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21870.1818621789034883No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21250.176706506707779No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21010.17471076263202054No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20920.17396235860361114No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20820.1731307985720451No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGG20160.1676425023637094No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20140.1674761903573962No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG19950.16589622629742073No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTTCTGTGCAG19670.1635678582090359No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCA18900.15716484596597755No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18840.15666590994703794No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC18750.15591750591862852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18630.1549196338807493No Hit
CCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA18610.1547533218744361No Hit
GTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAG18410.15309020181130406No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18150.1509281457292324No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18030.14993027369135317No Hit
GTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTT18020.14984711768819658No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG17980.14951449367557018No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17960.14934818166925698No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGG17800.14801768561875134No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGT17750.14760190560296835No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17550.1459387855398363No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17360.14435882147986084No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17140.14252938941041562No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16420.13654215718314028No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16330.13579375315473088No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16260.13521166113263466No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTC16160.13438010110106866No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16110.1339643210852856No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16090.1337980090789724No Hit
GACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT16050.133465385066346No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16000.133049605050563No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAGCTCCCAGGGGCGGCCCC15790.13130332898427438No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15710.13063808095902157No Hit
GGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC15430.1283097128706367No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15280.12706237282328767No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15050.12514978475068583No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15000.12473400473490281No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14770.12282141666230097No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC14740.12257194865283116No Hit
GATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAG14720.12240563664651796No Hit
GGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG14200.11808152448237468No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13800.1147552843561106No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG13590.11300900828982195No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG13470.11201113625194273No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13440.11176166824247293No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC13400.11142904422984652No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13290.11051432819512388No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13160.10943330015408807No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCT13130.10918383214461827No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13040.10843542811620885No Hit
CTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12920.10743755607832964No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG12830.1066891520499202No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12820.1066059960467636No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAAC12710.10569128001204099No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC12680.10544181200257119No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12680.10544181200257119No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG12530.10419447195522216No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTA12520.10411131595206556No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12510.10402815994890896No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12490.10386184794259576No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG12470.10369553593628256No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12300.10228188388262031No Hit
GTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGC12220.1016166358573675No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12210.10153347985421088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)