FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463432
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.044.5439831
GTATCAA14150.042.6379471
TATCAAC17100.035.2403452
TCAACGC17300.034.9904064
CAACGCA17400.034.785565
AACGCAG17500.034.7833026
ATCAACG17500.034.601723
ACGCAGA17950.034.1028827
AGAGTAC18400.033.08194411
CGCAGAG18600.032.7262238
CAGAGTA19100.032.2296210
GTGGTAT1850.031.6804871
TTTCGGG553.2736664E-431.33149131
GCAGAGT19950.030.5116679
CCGCAAT500.00744641527.81230444
TAGCGGC1403.583409E-1027.72082564
TAATCGT1254.0308805E-927.66744638
TTAATCG1254.0636223E-927.6464537
GAGTACT4500.027.51221512
ATAGCGG1305.0222297E-927.1032763
Produced by FastQC (version 0.11.5)