FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281945
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG200.007191928652.0411642
GTATCAA6400.041.9984551
GGTATCA2750.040.0992241
GTCGAAA350.001299138139.71423345
GAGTAAT350.00135373639.3831631
ATCAACG7650.034.68553
TCAACGC7700.034.460274
CAACGCA7850.034.240785
AACGCAG8000.034.0295146
TATCAAC8150.032.5575562
ACGCAGA8400.031.998827
CGCAGAG8650.031.4723858
TACGGGT1450.030.89541415
AGAGTAC8650.030.67561511
CAGAGTA8800.030.15273710
TGGTATC1301.9826984E-1029.1586912
CGCATTT500.007093684728.08630261
GTATTGT500.00776874127.5682141
GCAGAGT9650.027.4967969
GAGTACG7550.026.01636112
Produced by FastQC (version 0.11.5)