FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698968
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT120110.706958577206869No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCT92390.5438007072528734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90140.5305573736527115No Hit
GCTTAGGAGGCTGACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACT83540.4917102617589031No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC74990.44138559407828754No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC72990.4296137419892546No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG72080.4242575492887447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT71380.4201374010575832No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC70990.4178418899002218No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG63560.37410945938946466No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT60180.3542150293589991No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC60090.3536852960149926No Hit
GTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGA58910.34673990328246324No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATA57630.33920591794548216No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT55900.3290232658884688No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC54320.31972350273813277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG51690.30424351724105453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT51560.3034783468552674No Hit
GTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA46020.2708703165686464No Hit
GTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGG44920.26439579791967827No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44710.2631597534503299No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43560.25639093849913597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43520.2561555014573553No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC43380.255331471811123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43330.25503717550889715No Hit
CTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACAC43230.2544485829044455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG43060.25344797547687775No Hit
ATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT42500.2501518568919485No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA42300.24897467168304524No Hit
GTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAG41630.24503110123321922No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41490.24420707158698693No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39590.23302381210240566No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTA39430.23208206393528305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39390.23184662689350238No Hit
GTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT39350.23161118985172177No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA39220.23084601946593458No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCAC39120.23025742686148296No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT38020.2237829082125149No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA37930.22325317486850843No Hit
GACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA37840.2227234415245019No Hit
GCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATT37360.21989819702313407No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG37180.21883873033512108No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC36540.21507173766663057No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC35860.2110693079563594No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG35760.21048071535190774No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT35160.2069491597251979No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34240.20153410776424274No Hit
CCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCAT34080.20059235959712013No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG33910.19959175216955233No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA33510.19723738175174577No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC33170.1952361668966102No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33110.19488301133393918No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGAT32960.19400012242726172No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTT32580.19176347053034548No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT32410.19076286310277768No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA31710.18664271487161618No Hit
GGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG30590.18005047770175778No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA30550.1798150406599771No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC29920.17610690725193176No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG29850.1756948924288156No Hit
CTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC29690.17475314426169297No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAA29140.17151588493720893No Hit
GTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTGCTGGTA28960.170456418249196No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG28730.1691026552589572No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27930.16439391442334406No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA27870.16404075886067307No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27740.16327558847488594No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATT27560.16221612178687297No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCC27460.16162752918242135No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACT27340.16092121805707937No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26840.15797825503482113No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA26790.1576839587325953No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC26750.15744852169081466No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC26660.15691878834680817No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT26270.15462327718944677No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26220.15432898088722094No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC26180.1540935438454403No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCA25700.1512682993440724No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT25420.14962024005160782No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25320.14903164744715616No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC25080.14761902519647221No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25060.1475013066755819No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25000.1471481511129109No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAG24990.14708929185246572No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24770.14579438812267212No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24730.14555895108089148No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23840.14032047690127183No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT23630.1390844324319234No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23230.1367300620141168No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT23200.1365534842327813No Hit
GACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGT23080.13584717310743935No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT23030.1355528768052135No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22740.13384595825230375No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC22610.13308078786651661No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA22600.13302192860607145No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22420.1319624619180585No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22250.1309618544904907No Hit
CCACTGAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGT22230.13084413596960037No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21870.12872520259357445No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG21740.12796003220778732No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC21630.12731258034289053No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC21470.1263708321757679No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA21280.12525250622730974No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTACT20810.12248612098638703No Hit
GGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT20730.12201524690282571No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGC20630.12142665429837407No Hit
GTGTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTG20510.1207203431730321No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC20490.12060262465214176No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20170.11871912831789651No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGG20060.11807167645299971No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAAT19810.1166001949418706No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG19770.11636475790008993No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19620.11548186899341248No Hit
GTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGT19570.11518757269118665No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19500.1147755578680705No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACAT19150.11271548375248976No Hit
GGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGG19040.11206803188759293No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAG18820.11077312815779931No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18780.11053769111601867No Hit
GAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTG18700.11006681703245734No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG18690.11000795777201218No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18530.10906620960488957No Hit
ACCCGGGAATCGGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGC18320.10783016513554111No Hit
GGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCA18270.10753586883331528No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18110.10659412066619264No Hit
TTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCC18050.10624096510352167No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA17860.10512263915506354No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17650.1038865946857151No Hit
GCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACTCTGGCTGGACTT17640.10382773542526992No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG17620.10371001690437961No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17590.10353343912304411No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA17550.10329800208126345No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17300.10182652057013433No Hit
GTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC17240.10147336500746336No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG17120.10076705388212137No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGC17110.10070819462167623No Hit
GCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTC17010.10011960201722458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)