FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698968
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT121490.7150811551483018No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCT83900.49382919513492896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC74500.4385014903164745No Hit
GCTTAGGAGGCTGACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACT74370.43773631993068735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC72260.42531701597675764No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT71710.4220797566522736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC69590.4096015934378988No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG67880.39953665990177567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC62370.3671052073964901No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATA58120.34209002170729524No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC56190.3307301844413785No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT56130.33037702887870757No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC51090.3007119616143447No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA50080.2947671763093831No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG50020.29441402074671214No Hit
GTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGA49190.2895287021297635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49040.288645813223086No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT48380.2847611020337052No Hit
GTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGG48100.28311304274124055No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46900.27604993148782087No Hit
GTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA45670.2688102424530656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44980.2647489534823493No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43810.25786242001026505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43220.25438972364400037No Hit
GTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT42710.25138790136129696No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42420.2496809828083872No Hit
ATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT42380.24944554576660657No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC41640.2450899604936644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41130.242088138210961No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA40760.23991034557448992No Hit
CTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACAC39170.2305517231637088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38660.2275499008810054No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT38160.2246069378587472No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG37580.22119310075292767No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA37550.22101652297159216No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA37310.2196039007209082No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT37010.21783812290755328No Hit
CTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC36190.2130116635510498No Hit
GTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAG35880.21118702647724974No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35190.20712573750653338No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC34320.20200498184780408No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC34090.20065121885756532No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33880.19941517438821685No Hit
GACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA33520.19729624101219095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG33470.1970019447099651No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT32820.19317609278102943No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32800.1930583742601391No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTA32720.1925875001765778No Hit
GCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATT32260.18987997419610023No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGAT32240.18976225567520988No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC31850.18746674451784848No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT31830.18734902599695816No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA31820.187290166736513No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA31560.1857598259649387No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTT31470.18523009262093224No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCAC30640.1803447740039836No Hit
GGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG30630.18028591474353844No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30260.17810812210706733No Hit
GAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCC29310.17251649236477673No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC29010.17075071455142182No Hit
CCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCAT28470.16757231448738294No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG28140.1656299588926925No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27960.16457049220467954No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27380.16115665509886No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG27150.15980289210862123No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCC26790.1576839587325953No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA26640.15680106982591785No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA26220.15432898088722094No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA26160.15397582532454995No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACT26060.15338723272009833No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC25950.1527397808552015No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25850.15215118825074986No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC25690.15120944008362724No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC25670.15109172156273692No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25470.14991453635383362No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATT25280.1487962104053755No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAA25260.14867849188448518No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24910.1466184177689044No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24860.14632412146667859No Hit
GTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTGCTGGTA24760.14573552886222696No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24530.14438176587198817No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24070.14167423989151062No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC23580.13879013612969757No Hit
GCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGT23550.13861355834836206No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT23500.13831926204613626No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23440.13796610648346525No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA23040.13561173606565868No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT22870.13461112863809088No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCA22780.13408139529408442No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22730.1337870989918586No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22600.13302192860607145No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22150.13037326188603907No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAAT22080.12996124706292292No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAG22010.12954923223980674No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT21980.12937265445847126No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21770.1281366099891228No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21770.1281366099891228No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21590.12707714330110986No Hit
GACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGT21150.12448733584152262No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT21110.12425189879974195No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20990.12354558767439999No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG20810.12248612098638703No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20630.12142665429837407No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20390.12001403204769012No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC20250.11919000240145781No Hit
GTGTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTG20170.11871912831789651No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19850.11683563198365125No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19780.11642361716053509No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA19640.11559958751430281No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19410.11424582452406401No Hit
CCACTGAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGT19390.11412810600317368No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19260.11336293561738656No Hit
GCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCCGATTCCCGGGT18950.11153829854358646No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18930.11142058002269614No Hit
GAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTG18840.11089084667868965No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG18820.11077312815779931No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18180.1070061354893088No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18180.1070061354893088No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGC17780.10465176507150223No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACAT17720.10429860950883126No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG17600.10359229838348928No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17520.10312142429992795No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTACT17460.10276826873725697No Hit
TTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCC16990.10000188349633425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT2800.055.48063770
CGCTATA200.007400968651.6767432
GTGGTAT4000.049.0204851
TGGTATC4500.043.523682
GTATCAA35050.042.497421
GGTATCA13150.042.1175271
TATCAAC40650.036.347222
ATCAACG41000.036.002993
TCAACGC41050.035.866014
CAACGCA41250.035.604725
AACGCAG42100.034.967376
CTACGGA3200.033.04687569
CGCAGAG45500.032.3544248
ACGCAGA46200.031.8642047
AGAGTAC46650.031.63039411
CAGAGTA46850.031.49536510
GCAGAGT47800.031.0847789
GTGTCTA3000.028.33190365
ACGTGTC3000.028.21535363
TGTTAGA23700.027.58425370
Produced by FastQC (version 0.11.5)