FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491251
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCCGGAGTGGGTTG19080.3883961559365782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18850.38371423162497376No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15490.31531742429023046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14580.29679328897040413No Hit
GAGTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT14230.2896686215397017No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12880.26218776144984945No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12870.26198419952325797No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12370.2518061031936831No Hit
GTAATACACGGCCGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAAATACA12090.24610636924912113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11740.2389817018184187No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11630.2367425206259122No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11420.23246772016749076No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC11410.23226415824089924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11150.22697154814952028No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10820.22025400457200087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10800.21984688071881786No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10720.2182183853060859No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10650.21679345181994542No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10400.21170440365515797No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10000.20356192659149805No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9580.19501232567465512No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAA9160.1864627247578122No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA9140.18605560090462922No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9100.1852413531982632No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9070.18463066741848871No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9050.18422354356530574No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8750.1781166857675608No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG8700.1770988761346033No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8590.1748596949420968No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8530.17363832338254784No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8430.17160270411663284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8410.17119558026344983No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT8200.1669207798050284No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8120.1652922843922964No Hit
TTATTATAGTGTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC7990.16264597934660693No Hit
CTATAATAATTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7900.16081392200728345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7890.16061036008069193No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCGACCACTACATGGACTGGG7800.15877830274136845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7780.15837117888818547No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7740.15755693118181946No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC7680.1563355596222705No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7560.15389281650317252No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA7490.15246788301703204No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGTTTTCATTTGGTGAT7110.1447325298065551No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG7000.1424933486140486No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6980.14208622476086563No Hit
GTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAACCAGGGAAAGCCCCT6970.14188266283427414No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAATTTATTACTGCCAACACTATAATAATTAT6910.14066129127472515No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6870.13984704356835914No Hit
GTCCATGTAGTGGTCGCTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC6710.13659005274289518No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6710.13659005274289518No Hit
GTGTATTACTGTGCTATAGGCCTCTACGGTGGCTGGAACTACTATTACGA6660.13557224310993768No Hit
CAGTAATACACGGCCGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAAATA6610.13455443347698018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6470.13170456650469922No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6370.12966894723878425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6360.12946538531219276No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6330.12885469953241824No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA6300.12824401375264377No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTGCTAAACTAGA6230.12681908026650326No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6200.12620839448672877No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6090.12396921329422231No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6080.1237656513676308No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT6050.1231549655878563No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG5910.12030509861557534No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA5810.11826947934966035No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA5810.11826947934966035No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGC5620.11440180274442188No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA5580.1135875550380559No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT5580.1135875550380559No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG5520.11236618347850692No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA5440.11073768806577494No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC5430.11053412613918343No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG5380.10951631650622594No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5350.10890563072645146No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC5260.10707357338712797No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5260.10707357338712797No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG5250.10687001146053646No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5250.10687001146053646No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG5230.10646288760735347No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5160.105037954121213No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG5150.10483439219462148No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5150.10483439219462148No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5100.10381658256166398No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCA5050.10279877292870651No Hit
CGTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG5050.10279877292870651No Hit
GTATTACTGTGCTATAGGCCTCTACGGTGGCTGGAACTACTATTACGATA4960.10096671558938303No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4950.10076315366279152No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4920.10015246788301704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTC150.00235605168.99032613
TAATCGG200.006685802753.01621666
TTAAGAG200.00735173251.7585534
TGTCGCA200.00735173251.7585532
ATTAGTT408.2521E-751.75328422
GAATTAG408.2521E-751.75328420
AACGTTA200.007360615351.7427528
CAACGTT200.007360615351.7427527
ATATATT200.007360615351.74275214
TCTTGAG3550.049.68774870
GTATATA451.8557948E-646.0122831
AATTAGT451.8583996E-646.0029221
CAAAACG451.8610062E-645.99355314
CCTTTGT5150.043.6992770
GGTATCA4950.043.2236671
ACGTCCC503.540692E-641.88876356
TAATGGC503.793857E-641.47017332
TCATTAA503.8285707E-641.41527628
TTAGTTC503.8366197E-641.40262623
AAAACGA503.841995E-641.39419615
Produced by FastQC (version 0.11.5)