FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355340
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.042.4796371
TATCAAC12000.038.713522
CAACGCA12000.038.6699375
AACGCAG12050.038.2242246
ATCAACG12250.037.902093
TCAACGC12500.037.1283654
GGTATCA4700.036.6550061
ACGCAGA12850.035.8445057
CGCAGAG13400.034.3732768
AGAGTAC13750.033.49831811
CAGAGTA14000.032.90013510
GCAGAGT14750.031.2272479
GAGTACG12150.029.42239412
ACGGGAA1650.029.16520316
AGTACGG12350.028.94591713
TACGGGA4550.027.19643815
GTACGGG13650.025.68552614
AGTACTT4250.025.07227113
GTACTTT4450.024.71786114
ATAGAGT700.001359787924.5557964
Produced by FastQC (version 0.11.5)