FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750425
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23550.046.9830551
TCAACGC26650.041.12714
CAACGCA26800.040.8969155
ATCAACG26900.040.873013
AACGCAG27750.039.6210446
GGTATCA7800.038.8879621
TATCAAC28600.038.684522
ACGCAGA28900.038.1586047
AGAGTAC30350.036.44908511
CGCAGAG30350.036.3355378
CAGAGTA31150.035.73425710
GTGGTAT2700.034.468881
GCAGAGT32250.034.3016979
GAGTACG27750.033.40639512
AGTACGG27800.033.09838513
TACGGGA9050.031.22522515
TGGTATC3100.030.019282
GTACGGG30450.029.8783714
TACGGGG13100.026.04379315
TACGGGT5100.025.67755915
Produced by FastQC (version 0.11.5)