FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750425
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21550.042.884191
GGTATCA8500.040.5686721
ATCAACG25400.035.9143833
TCAACGC25450.035.5543444
TATCAAC25800.035.524152
CAACGCA26000.034.6699075
AACGCAG26000.034.6699076
AACGTTG700.002533425634.6680170
GTGGTAT2850.033.8784031
AGAGTAC27850.032.49041711
ACGCAGA28100.032.0789157
CTTATCG952.9288314E-431.93106370
CGCAGAG28550.031.6938048
CAGAGTA29050.031.148310
ATCGTAT450.004684271330.5824458
GCAGAGT30600.029.5705269
GAGTACG25350.028.77283312
AGTACGG25400.028.58073813
TAGCACC1005.135771E-727.52424
TACGGGT4500.026.7596415
Produced by FastQC (version 0.11.5)