FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607760
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6120.10069764380676582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.040.751541
GTATCAA16100.039.836431
GTGGTAT2750.035.109021
ATCAACG18750.034.1583563
TCAACGC18550.034.1441464
CAACGCA18700.034.051545
TATCAAC19400.033.0356252
GGGCGTT750.00341029332.6262970
AACGCAG20000.031.8381886
TGGTATC2900.030.8920462
GTCTAAC450.004632751530.6507321
AGAGTAC20900.030.63185311
GAACGAT450.004673993730.59525511
CGCAGAG20850.030.3751478
ACGCAGA21000.030.3220847
CAGAGTA21700.029.5025710
GCAGAGT23050.027.6253269
TACGGGT3950.027.01290115
AGTACGG20750.026.7063413
AACCGCT658.4964524E-426.62121239
Produced by FastQC (version 0.11.5)