FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279424
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38760.30294882697213743No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34290.2680112300535241No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG29320.22916562453103895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27710.21658183682657195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23640.18477064679105595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22680.17726727027162223No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA22470.1756259066579961No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21610.1689041318593367No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT20800.16257315792106447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20610.16108811465159323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20020.15647666449902456No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA19860.1552261017457856No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18290.14295495472962833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.13967222750237607No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC17460.13646766044720124No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT17190.1343573358011105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.13349757390825873No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG16750.13091828822970336No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG16720.13068380771347105No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15980.12489995497974088No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA15860.12396203291481166No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG15820.12364939222650193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15600.12192986844079835No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC15480.12099194637586914No Hit
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA15100.11802185983692663No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15030.11747473863238458No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14870.11622417587914562No Hit
CTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCA14120.110362162973338No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCGGTATATTACTGTCAGCATTATGGTGGTTCA13940.10895527987594418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13410.10481279075584013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13310.10403118903506577No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC13130.10262430593767195No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12930.10106110249612325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT200.00741012151.6598636
GTATCAA27800.043.485181
GGTATCA10400.039.0775761
CTTATAC3100.038.8852841
TATCAAC31450.038.436912
TATACAC3300.037.570813
TCAACGC32450.036.9340134
ATCAACG32600.036.7640763
CAACGCA32800.036.64495
AACGCAG33750.035.6134156
CTCGACG3250.034.99574328
AGAGTAC35750.033.52210211
CGCAGAG36100.033.1970948
ACGCAGA36350.033.063527
CGTATGC3250.033.0324141
TCGTATG3200.032.45102740
GCAGAGT37700.031.8795459
CAGAGTA38900.030.80758510
CGACGTC3700.030.75875330
TCGACGT3750.030.33913429
Produced by FastQC (version 0.11.5)