FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720208
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33970.4716692955368449No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29660.4118254726412369No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25020.34739964010396995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23580.327405416213094No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21710.30144069491035924No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21670.30088529980227935No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21290.2956090462755204No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG20600.28602848066114234No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG19090.26506231533112656No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19040.2643680714460267No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18940.26297958367582697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18630.25867527158820786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18130.25173283273720926No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA17490.24284651100793106No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17190.2386810476973319No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17020.23632061848799235No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16510.2292393308599738No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16490.22896163330593383No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16100.22354653100215496No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA15610.2167429409281763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15280.21216093128651722No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15160.21049474596227757No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14700.20410770221935887No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT14690.2039688534423389No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14290.19841490236154002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.19577677559816054No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT13940.193555195165841No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT13580.188556639193122No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.18827894163908204No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA13140.1824472930042432No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12840.17828182969364406No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG12810.17786528336258414No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12750.1770321907004643No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG12050.16731277630906627No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT12010.16675738120098638No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11970.16620198609290648No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA11850.16453580076866683No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11810.16398040566058694No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT11800.16384155688356697No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11720.1627307666674072No Hit
GTAATACACGCCCGTGTCCTCGACTCTCAGGCTGCTCATTTGGAGATACA11580.1607868837891276No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11320.15717681558660831No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11140.15467753760024883No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC11060.15356674738408904No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11020.15301135227600915No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA11000.1527336547219692No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT10920.15162286450580942No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10510.14593006464799058No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT10480.14551351831693068No Hit
ATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGAC10480.14551351831693068No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10460.14523582076289074No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT10380.14412503054673095No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10280.14273654277653122No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10280.14273654277653122No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10240.14218114766845133No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10170.14120920622931155No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10120.14051496234421168No Hit
ATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGT10080.1399595672361318No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10070.13982071845911181No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC10010.138987625796992No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTC9850.13676604536467243No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9810.13621065025659254No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9730.13509986004043276No Hit
GAAGTGGATCTGGGGCAAATTTTACCCTCACCATCAGCAGCCTGCAACCT9680.1344056161553329No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTCCTCC9640.13385022104725303No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9580.1330171283851332No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9560.13273943083109324No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9410.13065669917579364No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9110.1264912358651945No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9110.1264912358651945No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9080.1260746895341346No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8950.12426965543287495No Hit
CCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGAT8930.123991957878835No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8900.12357541154777507No Hit
GGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGG8870.12315886521671517No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8850.12288116766267522No Hit
CAGTAATACACGCCCGTGTCCTCGACTCTCAGGCTGCTCATTTGGAGATA8760.12163152866949548No Hit
ACCTTGACGGGACCCCTGGTTCCAATTTCGATGCATCGCAGATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTG8690.12065958723035568No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8620.11968764579121587No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTG8550.11871570435207607No Hit
GGTATCAGCAGCAACCAGGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGT8520.11829915802101616No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8500.11802146046697622No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8490.11788261168995623No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCT8480.11774376291293627No Hit
CTCCTGTGTAGTGTCTGGCTTCACCTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGTTCCAGGGAAAGGG8430.11704951902783639No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8390.11649412391975653No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8330.11566103125763669No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8300.11524448492657675No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8220.114133694710417No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8210.11399484593339701No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8130.11288405571723724No Hit
ACCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTGTTGACA8100.11246750938617733No Hit
CCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCG8080.11218981183213739No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8010.11121787039299759No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7930.1101070801768378No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC7810.10844089485259813No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCA7800.10830204607557817No Hit
GATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTTCAGCGGAAGTGGATCTGGGGCAAATTTTAC7780.10802434852153822No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7640.10608046564325861No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7640.10608046564325861No Hit
GCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAATAGTTGATG7600.10552507053517872No Hit
CCCTTAGACTCTCCTGTGTAGTGTCTGGCTTCACCTTTAGTGACTATTGG7590.10538622175815876No Hit
CCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTGTTGACAG7570.10510852420411881No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7460.10358118765689912No Hit
GCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGATCGG7440.10330349010285918No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7430.10316464132583919No Hit
GCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGA7420.10302579254881923No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7420.10302579254881923No Hit
GTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT7270.10094306089351965No Hit
CTATGTGGACTCTGTGAAGGGGCGATTCAGCATCTCCAGAGACAACGCCAAGAAAACACTGTATCTCCAAATGAG7250.1006653633394797No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7250.1006653633394797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT150.002315373269.2972741
GTATCAA11400.043.1791271
GTACCAC350.001362457839.3400233
GTGGTAT1400.039.3400231
TATCAAC12850.038.3067742
TCGTATG557.2136154E-637.7694840
CCCACGA557.4947166E-637.55966619
ATCAACG13400.036.734483
TCAACGC13750.035.799424
CAACGCA13850.035.5409435
AACGCAG13850.035.5409436
CACGGAA400.002624469634.422524
TAGGCAA902.0873008E-433.8274270
CGCAGAG14900.033.2674038
AGAGTAC15000.032.58665511
CAGAGTA15100.032.3708510
TGCCGTC652.2156932E-532.0617845
CGTATGC652.2531074E-531.98335841
CTCGTAT652.2747272E-531.93871939
GCAGAGT15350.031.8436379
Produced by FastQC (version 0.11.5)