FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677466
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10720.15823672331895625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2900.041.563551
GTATCAA18800.039.567491
GGTATCA8700.037.6051141
TGGTATC3500.033.4074552
TATCAAC22500.033.0144272
ATCAACG23000.031.9811343
TCAACGC22800.031.9507264
CAACGCA22850.031.8737495
AACGCAG23350.031.338366
TGCGAAC450.004717902330.53732512
ACGCAGA24550.029.8065437
AGAGTAC24600.029.46665811
CAGAGTA25450.028.6174910
CGCAGAG25500.028.561388
GCAGAGT26850.027.1253349
TACGGGG10050.025.63957215
GAGTACG23650.025.42087212
AGTACGG23800.025.11630613
ATAGCGG2300.024.58092563
GTATATG851.4600463E-424.3094371
Produced by FastQC (version 0.11.5)