FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483160
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22750.4708585147777134No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16280.33694842288268895No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15150.31356072522559814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11820.24463945690868452No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11160.23097938571073764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10780.22311449623313187No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10600.21938902227005547No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10340.21400778210116733No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10100.20904048348373208No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9610.19889891547313518No Hit
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACA9410.19475949995860584No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8900.18420399039655602No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT8480.17551121781604437No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8170.1690951237685239No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7760.1606093219637387No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7730.15998840963655933No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7670.1587465849822005No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAATATCACAGAACTCAGGCTGCTATCCCATGTT7420.1535723155890388No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC7400.1531583740375859No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7210.149225929298783No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAATATCACAGAACTCAGG6760.13991224439109198No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6610.13680768275519498No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6580.13618677042801555No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6490.13432403344647734No Hit
GTCTGGAGCCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGAAGCC6480.13411706267075088No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6390.13225432568921267No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6320.13080553025912742No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6310.13059855948340096No Hit
CTCACTGCACAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG6130.12687308552032453No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATCATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG5980.12376852388442752No Hit
GGGCATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTTCTGCGGAA5860.12128487457570991No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5740.1188012252669923No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5350.11072936501366007No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG5220.108038744929216No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5190.10741783260203659No Hit
GTGATGCCCAGGGTGGCTGACGTGCCAGACTTGAAGCCAGAGAATCGGTC5180.10721086182631014No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5160.10679692027485718No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5120.10596903717195133No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5080.10514115406904545No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5020.10389932941468663No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG4890.10120870933024258No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG4860.10058779700306317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA200.00665224753.08354666
TCGCGAA306.368636E-445.9104924
GGTATCA4700.043.215291
GTATCAA11100.041.5590021
CGTATGC856.184564E-1140.78793341
TCGTATG856.366463E-1140.76668540
ATCGCGA350.001360002239.3518523
GTGGTAT1400.039.3437041
TCTCGTA801.5970727E-938.9101838
GTCTTAT451.0134822E-438.250821
ACGAGAC901.2551027E-1038.2508222
ATCAACG12900.036.2900473
TCAACGC13250.035.3314444
CAACGCA13350.035.0667885
TATGCCG1003.5652192E-1034.69867743
TATCAAC13400.034.6790582
AACGCAG13650.034.5482676
TTAGCCC400.002623746234.4221764
TGGTATC1600.034.4221762
ATGCCGT1055.984475E-1033.07050744
Produced by FastQC (version 0.11.5)