FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406437
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12090.29746307545818906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.039.717271
ACGGGTA350.001356905939.36860316
GTATCAA9600.038.853851
TAACGGC400.002573050334.5580736
TTAACGG400.00257772734.5452835
ATATCGC400.002613805234.447536
TCAACGC11600.032.372784
CAACGCA11650.032.233845
GACGAAA450.00404377431.51171766
ATCAACG11600.031.4895343
AACGCAG12150.031.4705816
TATCAAC11650.031.369832
ACGCAGA12200.031.3416047
AGAGTAC12350.030.68200911
TATATCG450.004650933730.6237895
TTATACC450.004650933730.6237894
CGCAGAG12500.030.5894058
CAGAGTA13100.029.4513210
TCGGCAC605.130943E-429.0168650
TTAGGTG605.4097053E-428.75580229
Produced by FastQC (version 0.11.5)