FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21269
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT6563.0843010954910905No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1030.48427288542009495Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT910.4278527434294043Illumina PCR Primer Index 10 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.15515539047439936No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.15515539047439936No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.14575203347595092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.13634867647750246No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.12694531947905402No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11754196248060557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11284028398138135No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC220.1034369269829329No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG220.1034369269829329Illumina PCR Primer Index 10 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)