FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21269
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11015.17654802764587No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGACAAAAA240.11284028398138135No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1034369269829329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGC150.002144077370.40246643
TTGCGCT150.002144077370.40246644
GTGCTTC150.00234009768.8719834
AAAACGG150.00250155867.72684526
AACGGTG150.00250155867.72684528
CCGTGAA150.002597469867.089429
GATACCG150.002597469867.089425
GAAGCCA150.002597469867.0894217
GAAAAAC150.002597469867.0894224
TGTTTGT150.002597469867.089422
AGAAAGA150.002597469867.0894219
ATACCGT150.002597469867.089426
AAAGAAA150.002597469867.0894221
GGGAAAT300.00521683756.12795370
ACGCAGG200.0054236255.68945365
CAAACGC200.00582710754.6924662
ACAAACG200.005886520754.55293761
TACAAAC200.005946384354.41412760
CTATACA200.006067469754.1386157
TATACAA200.006067469754.1386158
Produced by FastQC (version 0.11.5)