FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709439
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28270.398483872468246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21910.3088355728963308No Hit
GTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG21510.3031973150616191No Hit
GTTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACACT18960.2672534213653323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18860.2658438569066544No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18350.2586550781673971No Hit
CTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT18130.25555403635830565No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17270.2434317820136756No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGA17190.24230413044673327No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCA17150.24174030466326213No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16930.23863926285417067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16640.23455152592400472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16530.23300100501945903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14770.20819267054672774No Hit
GTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC14270.2011448482533382No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA13780.19423798240581644No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13520.19057311481325384No Hit
GTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGC13290.18733111655829465No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12860.1812699893859796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12580.1773232089016815No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12570.17718225245581368No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12490.17605460088887134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12310.1735173848632511No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT12230.17238973329630877No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA12150.17126208172936644No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12080.17027538660829192No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11950.1684429528120106No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11920.16802008347440725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11730.1653419110029192No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11720.16520095455705142No Hit
GACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAA11610.16365043365250573No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACAC11420.16097226118101768No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11410.1608313047351499No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10900.15364252599589254No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAA10880.15336061310415694No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10750.15152817930787565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10730.15124626641614008No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGGAGTTTAGGAGGCTGCCCTGGTCTCTGCTGGTA10200.14377557478514713No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10190.14363461833927935No Hit
GCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATT10170.14335270544754375No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAA10050.14166122809713028No Hit
GCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTG9720.13700966538349316No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9500.13390862357440173No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCC9430.1329219284533272No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC9110.12841132218555787No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9060.12770653995621892No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8960.12629697549754101No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8900.12545123682233425No Hit
CTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTATTGACCCAGTCTCCAGACTCCCTGGCTGTGT8730.12305497724258183No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT8670.12220923856737506No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCACTTCTCTGTT8640.12178636922977171No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA8310.11713480651613457No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACACCTCCCA8300.11699385007026679No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGAT8280.1167119371785312No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTAC8110.11431567759877875No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7880.11107367934381955No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAA7830.11036889711448059No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7800.10994602777687722No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7600.1071268988595214No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7560.10656307307605024No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7490.1055763779549757No Hit
GTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACACCTCCCACAGTAAGAACTA7480.1054354215091079No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC7430.10473063927976893No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7430.10473063927976893No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGC7270.10247533614588429No Hit
ATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCAT7210.10162959747067753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12850.043.174721
TATCAAC14250.038.6911742
ATCAACG15350.035.918523
GGTATCA5200.035.7846871
TCAACGC15450.035.686044
CAACGCA15800.034.8955235
AACGCAG16450.033.726156
ACGCAGA17250.032.1597757
CGCAGAG17650.031.2357148
AGAGTAC17750.031.05974211
GTTATAG450.004648254730.6305123
CTCGATA450.004649857530.62835512
CGATTGT800.00486579530.3422670
GCAGAGT18400.030.1497889
TGTTAGA6450.030.1070570
CAGAGTA18500.029.98681610
GTGGTAT2250.029.0989911
AGCAACG500.00777759227.5674655
TGCTAAT900.00864920926.97089870
AGTACGG16550.026.64944513
Produced by FastQC (version 0.11.5)