FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770149
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.042.1713561
GGTATCA9350.039.792331
TCAACGC26600.036.9081464
CAACGCA26950.036.428825
ATCAACG27050.036.16683
AACGCAG27550.035.6354526
TATCAAC27600.035.5708962
ACGCAGA29250.033.564337
AGAGTAC30400.032.40794411
CGCAGAG30200.032.3944328
CAGAGTA31050.031.72951710
GTGGTAT2950.031.5303631
CGTATAC450.004656241730.6200923
GCAGAGT32300.030.5015959
AGTACGG25750.029.0296313
GAGTACG26050.028.82755512
TTAACGG2800.028.39765535
TTTAACG2850.027.89036234
GCGTATA500.00779092727.5580832
TAACGGC2900.027.436336
Produced by FastQC (version 0.11.5)