FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770149
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24550.044.0896071
GGTATCA9350.042.7665751
TCAACGC29500.036.6033744
TATCAAC29850.036.2330062
ATCAACG29850.036.192993
CAACGCA30000.035.878695
AACGCAG30400.035.745966
ACGCAGA32200.033.8545347
AGAGTAC33300.032.32314711
CGCAGAG33800.032.2519578
CAGAGTA33950.032.2107510
GCAGAGT35150.031.0132629
CGATTTA450.004689542630.57551418
ACGGGTA2050.028.52101316
GTGGTAT2850.027.8189681
AGTACGG30350.026.7420713
GAGTACT9800.026.66864812
GAGTACG30850.026.6413512
TACGGGT5900.026.23023415
AGTACTT10100.025.19718613
Produced by FastQC (version 0.11.5)