FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344220
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.045.364621
ATCGACT350.001243449740.07212459
GTATCAA8000.038.786751
TATCAAC9600.031.9492242
ATCAACG9950.030.8253843
TCAACGC10000.030.6712574
TTGTATG450.00464314830.6329634
CAACGCA10200.030.0698595
ACGCAGA10450.029.3504857
AACGCAG10750.028.53146
GCGACAT600.00857357527.01708269
AGAGTAC11150.026.88969811
CGTCAGA658.700405E-426.5092965
CGCAGAG11550.026.2568268
CAGAGTA11800.025.70053910
TGGTATC1101.1599186E-625.0633352
GCGTCAG700.001340511324.6157764
GTACGGA700.001340511324.61577614
GCAGAGT12500.024.2613099
AGTACGG10300.023.75541713
Produced by FastQC (version 0.11.5)