FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344220
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3700.10748939631630933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.042.3621981
GGTATCA3250.041.420821
AACGCGA400.002632125734.3970076
TATCAAC10750.034.3117872
TCAACGC11400.032.6103254
ATCAACG11600.032.071393
CAACCGT450.00382713731.86663267
AACGCAG11500.031.7050676
CAACGCA11900.030.9373875
CGCAGAG11900.030.639358
ACGCAGA12000.030.3840247
GTGGTAT1501.8189894E-1229.9150351
AGAGTAC12400.028.84910411
CAGAGTA12650.028.55087710
ATAGCGG755.331818E-528.18497563
GCAGAGT13000.028.0467919
ACGGGTA500.00782996427.5256025
GCTATCA500.00784107727.5176078
TGGTATC1404.6929927E-1027.0852362
AGTAGTC657.7479443E-427.04058561
Produced by FastQC (version 0.11.5)