FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386396
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7230.1871137382374559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAT350.006847521852.6869270
GTATCAA9700.041.926711
CTTGCGT1350.035.83318768
ATCAACG11050.035.4923863
CAACGCA11050.035.4786035
TCAACGC11100.035.3279344
AACGCAG11250.034.8478746
AGAGTAC12050.033.96126611
GGTATCA4100.033.6245571
TATCAAC11700.033.550992
ACGCAGA11800.033.223617
CAGAGTA12450.032.5939210
CGCAGAG12400.031.8933478
GTATCAC553.3073727E-431.2751873
GAGTACG11100.030.67158112
AGTACGG11000.030.63778313
GCAGAGT13500.029.8041029
CGTTCGG703.908888E-529.4994921
CGGGCGA850.006140077528.92615370
CCGTAGC500.007712893727.61153235
Produced by FastQC (version 0.11.5)