FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655505
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAACTCCTCATCTATGGTAACAACAATCGG21240.324024988367747No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGT20130.3070914790886416No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTG18580.2834455877529538No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18430.28115727568820986No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGA16900.2578164926278213No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGG14390.21952540407777207No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14360.21906774166482332No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC13660.20838895202935143No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13370.20396488203751306No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13100.19984592032097392No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12930.19725249998093072No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12930.19725249998093072No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12090.18443795241836447No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12060.18398029000541566No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11960.18245474862891967No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG11920.1818445320783213No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10880.16597890176276306No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10800.16475846866156627No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10660.16262271073447188No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT10390.15850374901793274No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC10310.15728331591673594No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC10020.15285924592489758No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT9930.1514862586860512No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9920.15133370454840162No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9910.151181150410752No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9230.1408074690505793No Hit
GTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG9190.14019725249998094No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGG9150.13958703594938254No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9020.13760383215993777No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8750.1334848704433986No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8670.1322644373422018No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8560.13058634182805623No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC8440.12875569217626104No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8440.12875569217626104No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8220.12539950114796988No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT8010.12219586425732831No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7990.12189075598202913No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT7930.12097543115613153No Hit
GGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAG7930.12097543115613153No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7880.12021266046788354No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7790.11883967322903716No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7720.11777179426548996No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7580.11563603633839559No Hit
GTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGT7510.1145681573748484No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT7460.11380538668660041No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG7240.11044919565830924No Hit
GTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTT7120.10861854600651406No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6950.10602512566647089No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT6930.10572001739117169No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC6800.10373681360172692No Hit
AGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAAC6780.10343170532642772No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC6730.10266893463817972No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC6610.10083828498638454No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG6590.10053317671108535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.042.335611
GTGGTAT1850.039.1918261
TCAACGC18100.039.1810154
ATCAACG18200.038.977633
TATCAAC18500.038.7711072
CAACGCA18600.038.121955
AACGCAG18600.038.121956
ACGTCAC655.273032E-737.3075841
ACGCAGA19450.036.4559447
AGAGTAC19850.035.54791611
CGCAGAG19950.035.5422638
CAGAGTA20050.035.5366710
AGTTTCG750.002621438434.4257170
GGTATCA6600.034.0030021
AGTACGG17200.032.4195813
CGTTAAA652.3530674E-531.78030817
GAGTACG17750.031.60894612
GCAGAGT22750.031.3191349
TTAGTAC450.004663058630.6102643
GTGCTAA605.3957175E-428.7717721
Produced by FastQC (version 0.11.5)