FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306334
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG150.002450793568.301554
TACGTTA200.00697748952.44196347
GGTATCA7350.040.5159761
ATTTCGC654.193862E-738.40159662
GTATCAA16850.038.3933751
CGTTGAG557.78667E-637.3407441
TATCAAC18800.034.3885732
ATCAACG19250.033.363343
TCAACGC19550.032.665964
CAACGCA19650.032.4944155
AACGCAG19650.032.4944156
GTGGTAT3300.032.1545261
ACGCAGA19900.032.0861937
TGGTATC3300.031.096932
AGAGTAC20350.031.04109211
CAGAGTA20550.030.7389910
TACATCG450.004856922730.351295
ACGATCA450.004856922730.351299
GCAGAGT20950.030.315079
TTACCCG703.4807606E-530.00193648
Produced by FastQC (version 0.11.5)