FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297020
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA300.00427653759.33739570
CGAATTT252.5059443E-455.45672242
GTGGTAT1350.045.87161
GTATCAA8600.040.8043941
GGACGAA350.001248114940.03977254
TCAACGC10000.035.0917784
CGGGTAA400.002629036834.403718
ATCAACG10300.034.40373
CAACGCA10300.034.0696875
TATCAAC10450.033.909872
AACGCAG10750.032.9635476
ACGCAGA11150.031.7817
GGTATCA3850.031.2760941
TGGTATC1800.030.581072
CGCAGAG11700.030.2870228
AGAGTAC12200.029.32774711
CAGAGTA12300.029.08931210
GCAGAGT12850.027.576519
TTACGAC500.00778057927.56008331
TTCTAAC500.0078319327.5229642
Produced by FastQC (version 0.11.5)