FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297020
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8300.041.9639471
AACGCAG9000.037.0485236
GTGGTAT1400.036.9484521
TATCAAC9350.036.8328252
CAACGCA9100.036.641395
TCAACGC9300.035.8594444
ATCAACG9400.035.501873
ACGCAGA9600.034.3749167
GGTATCA3250.033.954681
AGAGTAC10300.033.03996711
TACGGGA3000.032.08325615
CGCAGAG10350.031.8839788
GCAGAGT10550.031.6053759
CCGTGAT450.00421047831.24992659
CAGAGTA10800.031.19205310
GAGTCGC450.004424021630.93566546
ATACTAC450.004692259730.56577122
TAGCAAT450.00469999830.55548111
AGTACGG10050.030.44146713
GAGTACG10250.029.84748612
Produced by FastQC (version 0.11.5)