FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831484
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.040.3849871
GTATCAA24350.037.831911
GTGGTAT4050.037.4838031
TACACGG400.002596666834.4979862
ATCAACG28550.032.141733
TCAACGC28750.031.9181394
TATCAAC29150.031.3618072
AGGTACG450.00415494331.34154366
CAACGCA29500.031.22365
AACGCAG30100.030.6012026
TGGTATC5100.029.0865362
ACGCAGA31800.029.0720257
AGAGTAC32000.028.99812511
CAGAGTA32650.028.63213310
GCAGAGT33400.028.0924789
CGCAGAG33100.028.0344438
GTTATCG500.00769286727.62996130
AGTACGG28850.025.94822513
GAGTACG29200.025.6356612
TTAACGG2450.023.99944135
Produced by FastQC (version 0.11.5)