FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831484
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23100.040.090761
GGTATCA9850.036.4853551
GCGATAA400.00239039335.08505661
ATCAACG26800.033.6947823
TATCAAC27350.033.4254232
TCAACGC27200.033.18134
AACGCAG27700.032.7008176
CAACGCA27900.032.4664045
ACGCAGA29850.030.3454867
CGCAGAG30550.029.650178
AGAGTAC31150.029.4107611
CAGAGTA31200.029.36362610
GCAGAGT33600.027.2662269
GTATTAG3600.026.8767661
GTGGTAT3800.026.3715631
CCGTTAA1457.1486284E-1026.13121416
AGTACGG28450.025.42258313
GAGTACG29000.025.05919812
ATAGGAC700.00134112424.618873
GCCGTTA1653.1996024E-922.96241415
Produced by FastQC (version 0.11.5)