FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780448
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24550.041.9181561
GGTATCA10100.038.8477551
GTGGTAT3550.037.811051
TCAACGC28350.036.4185684
CAACGCA28700.036.094355
ATCAACG28850.035.906693
AACGCAG29050.035.541016
ACGCAGA30050.034.2393657
TATCAAC30650.033.7979742
AGAGTAC30950.033.24584611
CAGAGTA31450.032.60787210
CGCAGAG32000.032.045378
TGGTATC4100.031.8973352
ACGATTG553.0730417E-431.6745247
ATCGGAT450.0044166330.9525746
GCAGAGT33400.030.8051789
AGTACGG25900.029.63191813
GAGTACG26350.028.99340212
CGATCGC500.00782891327.53067219
CTCGCTA1005.1285315E-727.52890823
Produced by FastQC (version 0.11.5)